An Exclusive Weber Product: Lumitester™ Smart: The A3 Sanitation System (Kikkoman)

Better detection equals better protection

Find what others can’t - See for yourself why there’s no clean like A3 clean
Only Kikkoman’s new A3 Sanitation System, distributed by Weber Scientific, detects ATP+ADP+AMP with one swab to give you the whole picture. Its patented A3 Technology has been proven to detect residues and microorganisms that others miss.

  • Hygiene monitoring in three easy steps
  • Visualize the data with App
  • Access from anywhere with cloud storage

ATP+ADP+AMP with A3 Detection

MEASURE: Easy-to-use, results within 10 seconds

Step 1: Swab — Swab the sample and insert the swab into main body

Step 2: Measure — Shake well and insert into the Lumitester

Step 3: Analyze — Measurements (RLU) displayed within 10 seconds

UNDERSTAND: App to continuously monitor multiple data

Recorded data can be displayed in time-series by registering with the specialized App. Inspection pass rates are automatically graphed and improvements can be visualized. Employees will be more conscious of hygiene, and will maintain a high standard of environmental sanitation, which will help build greater trust in your business.

CONNECT: Centralized multi-site data with cloud data management

Data will be stored in the cloud and can be accessed from anywhere in the world. This will allow fast detection and response to any problems that may arise.

The proof is in the testing

Independent laboratory testing confirms that the Kikkoman A3 technology provides detection of residues at levels much lower than other tests on the market. Using residue from many different foods, laboratory testing proved that Kikkoman A3 technology produced a test result (relative light units - RLU) an order of magnitude or higher than competitive products. In many of the foods tested, Kikkoman A3 technology showed a strong presence of residue while the competitive products produced test results below typical action levels showing that Kikkoman A3 can reduce the risks from false negative results.

AOAC approved

Eliminate Contamination
Sanitation is critical to food safety and ineffective cleaning can affect the appearance and taste of food, harbor microorganisms and promote the production of biofilms. So, if your sanitation system only detects ATP, you’re missing potential contaminants.

ATP can be unstable and decompose into ADP and AMP. This means conventional systems that test for ATP alone may fail to find the true presence of contamination and may produce false negatives. Only the breakthrough chemistry in Kikkoman A3 technology detects ATP, ADP and AMP - more than any other product out there. Ensure better surface sanitation and support a more effective sanitation program that reduces the presence of resident organisms and the risks from food pathogens.

Detection - A3 vs. Competitors
ATP in meat, seafood, egg and fruit is decomposed into ADP and AMP during processing increasing levels of ADP and AMP. The A3 Sanitation System showed superior sensitivity and stability for detection of food residues compared to competitive ATP tests.

Find out what you’ve been missing and receive our FREE white paper.

The target foods were homogenized and diluted 100-fold, then aliquots of 10 µl of each dilution were pipetted onto the appropriate swabs and measured.

Use the Lumitester Smart A3 Sanitation System for comprehensive allergen residue control

The A3 Sanitation System has ultra-sensitivity which makes it ideal for highly effective allergen control by the food and beverage industries. A pass from the Lumitester Smart ensures there is no cross-contact from allergenic food residues on all processing surfaces and equipment. It also works with rinse waters. A3 Sanitation System is much better than traditional ATP bioluminescence because it is the only technology that measures ATP+ADP+AMP (adenosine triphosphate + adenosine diphosphate + adenosine monophosphate) and detects any residues at much lower levels than was previously possible, and at levels comparable or lower than allergen specific test kits! All-in-all a terrific allergen test.

  • Results in 10 seconds using the Lumitester - no incubation necessary!
  • Effective detection of all food allergens in one test
  • Prevents cross-contamination by verifying the cleaning process
  • Allows for immediate corrective action to be taken when necessary
  • Simple to use all-in-one test device

Superior sensitivity leaves food residue with no place to hide


  • Highly cost effective sanitation monitoring
  • Provides real-time feedback and facilitates long-term data analysis
  • Optimizes and verifies that cleaning procedures are working
  • Assists in developing and improving process and risk assessment programs (i.e. HACCP & GMPs)


  • No scheduled yearly maintenance necessary
  • Available Calibration Kit for in-house confirmation of calibration
  • Powerful software included, connects to software via USB
  • Small, lightweight, handheld instrument (0.57 lbs., 7 x 18. x 3 cm)
  • Quick-test mode enables ad-hoc testing
  • Large screen with back-light and optional BOLD text
  • Powered by 2 x AA batteries for several months of uninterrupted use
  • Removable read chamber design allows for easy cleaning
  • Durable outside shell and sealed keypad to protect against splashes and wear
  • Original one year warranty includes parts and labor (additional coverage available)

Common Threshold Setting

  • Food contact soft surface 500-1000 rlus
  • Food contact stainless surface 200-400 rlus
  • Food service hands 1500-3000 rlus
  • Hospital 1000-2000-rlus
  • Total ATP Water 200-500 rlus

Measurement Principles
ATP is a source of energy necessary for various forms of life that are present in organic residues, such as microorganisms, food residue, and biological substances that originate from other living organisms. This monitoring system allows you to measure and detect organic residues at high speed and high sensitivity by detecting ATP (adenosine triphosphate) using luciferase, which is why it is widely used in determining cleanliness levels in food manufacturing facilities. However, conventional ATP monitoring systems are insufficient because ADP (adenosine diphosphate) and AMP (adenosine monophosphate) generated from ATP degradation are completely overlooked. Kikkoman developed a new and patented monitoring system. This method definitely enables high sensitive analysis of a wider range of organic residues.

Measure Methods

  1. The swab devices should be allowed to reach room temperature prior to testing, if they are from refrigerated stock.
  2. Remove the swab stick from the casing.
  3. NOTE: If running the test on a dry subject, first moisten the swab or the test subject with tap (or deionized water for consistency)1.
  4. Use the swab with consistent pressure.
  5. Return the swab stick to the casing and push all the way into the main body.
  6. Allow the leftover luminescent reagent to thoroughly dissolve.
  7. Insert the swab into the Lumitester™ to measure the results.
    1Regular tap water may contain a slight amount of ATP, ADP and AMP. It is recommended to wash or otherwise clean off the faucet in such cases and allow the water to run for a while before collecting any for use. Alternatively use deionized water.

Handling of Data
Benchmark levels 1 and 2 are set to determine whether cleanliness levels are at acceptable levels or not (see diagram). Cleanliness levels are designated as Pass (A) if the measured amount of luminescence falls below level 1 and as Fail (C) if the amount is higher than level 2. With Fail (C), cleaning will need to be redone and cleaning procedures may need to be revised. Cleanliness levels where the measure amount of luminescence is above level 1 but below level 2 are designed as Caution (B), and it is recommended that cleaning be redone and cleaning procedures revised in such cases.

Powerful Data Analysis Software package is included (see illustration)

  • 100 programmable test plans
  • 200 programmable user IDs
  • 251 programmable locations per test plan
  • 5,000 programmable test locations

The Repeatability of Measurements
The Lumitester Smart with LuciPac A3 swab demonstrated superior repeatability relative to competitors even at the low level of ATP (10-13 moles ATP/assay). (See chart.)

ATP detection was assessed for each device by pipetting 10 μL of the 10-8 M stock ATP solution (10-13 moles ATP/assay) onto the appropriate swabs (n=10)

Store at cool temperatures (36° - 46°F - 2° - 8°C) for long term storage. The kits can be stored below 77°F - 25°C for up to 14 days or below 86°F - 30°C for up to five days before opening an aluminum bag, without any adverse effect on the long term stability. Do not freeze the kits.

Storage Stability
The RLU of LuciPac A3 was decreased only 8% after extreme storage at 40ºC for 2 weeks (see chart).

Each device was stored at 4ºC and 40ºC respectively, the sample stored at 4ºC served as a control (100 RLU). 10 μL of the 10-7 M stock ATP solution (10-12 moles ATP/assay) was used for evaluations.


  1. This kit cannot be used to test food or food products directly.
  2. Set the benchmark level at which the required cleanliness levels can be obtained. If the benchmark level is not set correctly, cleanliness levels may not be evaluated correctly.
  3. See storage recommendations.


Lumitester Smart Instructions for Use
Lumitester Smart Quick Start Manual
Lumitester Smart Brochure
LuciPac A3 Surface Swab Instructions
LuciPac A3 Water Swab Instructions

Item No. Alt. Part No. Product Description Packaging Unit of Measure Your Cost Purchase
Lumitester™ Smart
EF43052 Lumitester Smart: ATP + ADP + AMP with A3 Detection Each
Buy 1 $1,448.99/EA
Buy 2 $1,241.99/EA
Add to Cart 25 In Stock 25 In Stock
LuciPac™ A3 Surface Swab System
EF40948 LuciPac A3 Surface Swabs 20 x 5 Aluminum Bags Pack of 100
Buy 1 $258.56/PK
Buy 2 $240.53/PK
Add to Cart 40 In Stock 40 In Stock
LuciPac™ A3 Pre-Moistened Surface Swab System
LuciPac™ A3 Pre-Moistened Surface Swab System 20 x 5 Aluminum Bags Case of 100
Buy 1 $258.56/PK
Buy 2 $240.53/PK
Add to Cart This item is on back-order. Allow 5-10 business days. Please call us at 800-328-8378 with any questions about availability. In Stock
LuciPac™ A3 Water Swab System
EF40949 LuciPac™ A3 Water Swab System for TOTAL ATP+ADP+AMP - layered flange tip for highly accurate water sampling 20 x 5 Aluminum Bags Pack of 100
Buy 1 $258.56/PK
Buy 2 $240.53/PK
Add to Cart 24 In Stock 24 In Stock
Lumitester™ Calibration Kit
EF40950 Lumitester Calibration Kit 20 x 5 Aluminum Bags Each
Add to Cart 1 In Stock 1 In Stock