SpotCheck™ Plus Glucose and Lactose Residue Test

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SpotCheck Plus glucose/lactose device, for dairy products
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SpotCheck Plus detects the presence of glucose and lactose on a surface. Simply swab a surface, release the reagent and if any residue containing glucose and/or lactose is present the reagent will turn green, indicating poor surface hygiene. The more contamination present, the quicker the color change and darker the color. SpotCheck Plus instantly validates the hygiene of a surface, allowing immediate corrective action to be taken if necessary.

SpotCheck Plus is an extremely convenient all-in-one swab device. Unlike ATP bioluminescence hygiene-monitoring systems, it requires no expensive instruments. Simply swab, snap, squeeze and read a definitive color change - “if it turns green it isn’t clean.” By testing with SpotCheck Plus regularly you can minimize the risk of foodborne hazards as well as maintain the high quality and long shelf-life of your products. And since SpotCheck Plus measures simple sugars, you can detect all major food groups. This is a great advantage over swabs that only detect protein residue, which typically are limited to high protein foods such as meat.


  • All-in-one device
  • Easy-to-interpret color results
  • No instrumentation necessary
  • Results in 60 seconds
  • Pre-moistened swab for reliable collection, recovery, and detection
  • 100% recyclable
  • 18 month shelf life at refrigerated temperatures (2-8°C)
  • 4 week shelf life at room temperatures (21-25°C)


  • Detects down to 2.5 μmoles D-glucose or 5.0 μmoles lactose in 60 seconds
  • Disregard any color change after 2 minutes

SpotCheck Plus, made by Hygiena, is ideal for rapid cleaning validation and sanitation monitoring. Incorporate SpotCheck into your HACCP plan.
• analyze hazard – bacteria growth on food residues
• determine CCP’s (critical control points) – removing residue
• set criteria – establish cleaning requirements
• monitor – test with SpotCheck
• set corrective action – additional cleaning
• verify – test with SpotCheck
• documentation – record results

• dairy processors and on-farm
• food processing plants
• health departments, inspectors, auditors, consultants
• restaurants
• delicatessens and convenience stores
• supermarkets

Products detected
• dairy products (except fermented)
• meats (raw and cooked)
• fruits
• root vegetables
• salad vegetables
• food dressings and sauces
• beverages (except low calorie)
• cooked and prepared foods
• bakery products (except sucrose only products)

Precautionary note:
Hygiene monitoring tests should be performed post-cleaning and pre-sanitization to verify cleaning effectiveness. The manufacturer recommends that if the SpotCheck device is used post-sanitization, then the sanitizer must be given time to dry completely before the sample is taken. Of particular concern are peroxide-based sanitizers, which can give a false-positive result if not left to dry before sampling. You’re welcome to call the Weber Scientific Technical Service department for additional information.

Industrial Food Processing - Lowest Dilution Detected

Sample Type Commercial
Protein Test
SpotCheck Plus ATP
Dairy Products 1/10 1/100 1/100 - 1/1000
Meat 1/10 1/10 1/100
Root Vegetables Not detected at all 1/10 1/50
Salad Vegetables Not detected at all 1/100 1/100
Fruits Not detected at all 1/500 1/500
Bakery Products Not detected at all 1/10 1/100

Food Service/Catering - Lowest Dilution Detected

Sample Type Commercial
Protein Test
SpotCheck Plus ATP
Salad Vegetables
Lettuce Not detected 1/10 - 1/100 1/1000
Tomato Not detected 1/10 1/10000
Pickle Not detected Not detected 1/100
Salad dressing 1/10 ? 1/10 - 1/100 1/10
Ketchup 1/10 ? 1/1000 1/100
Cola Not detected 1/100 Not detected
Milk Shake 1/10 ? 1/100 1/100
Cooked Products
Beef 1/10 ? 1/10 ? 1/1000
Bacon Not detected 1/10 ? 1/10
Chicken 1/10 ? 1/10 1/10000
Egg 1/10 ? 1/10 1/10
French Fries 1/10 ? 1/10 1/100
Bakery Products
Bread bun 1/10 1/100 1/100
Apple Pie 1/10 ? 1/100 1/100

Products identified by this icon indicate only the first case — of multiple case orders — will be upgraded to UPS 3 Day Select service (where applicable). The remaining cases will ship by ground and you should receive them within 1 - 3 days of receiving your first case.