Phosphate Buffers | See Why and How to Use Phosphate Buffers

Phosphate Buffers: Here’s What You Need to Know

One of the most important things to understand is that often research efforts are only as successful as the products those hardworking men and women are using to conduct their work in the first place.

Such is the case with phosphate buffers, also commonly referred to as phosphate buffer saline (and usually abbreviated as PBS). This is a type of buffer solution used by biological researchers all over the country that is made up of sodium chloride, sodium phosphate, and sometimes potassium chloride and potassium phosphate.

Phosphate buffers are widely used because they help maintain a constant pH level in a particular environment. Generally speaking, most researchers try to maintain a pH of 7.4 as often as possible because the properties closely match those of the human body.

However, phosphate buffers have a wide range of additional uses, too. A large part of this has to do with the fact that the solutions are both non-toxic (with regards to most cells) and isotonic, too. Sometimes they're used to dilute certain substances so that they're safer to work with. Often, these solutions are also used to rinse containers that previously held cells and other materials. Many labs and their employees use it as a way to dry bio molecules as well.

The Many Uses of Phosphate Buffers

How you will use phosphate buffer solutions ultimately depends on what your application is and what type of product you happen to be using. Buffer Dilution Water Pillows, for example, are made from magnesium chloride and potassium dihydrogen phosphate and are designed to create 25 L of dilution water at a ratio of one liter of distilled water per pillow. This particular product is recommended for diluting most nonpotable and wastewater samples you happen to be working with and comes in a pack of 25 pillows.

All told, phosphate buffers are an important part of many fields and play a critical role in the food services industry, in particular. This technique is commonly used to test certain types of foods for bacteria that could ultimately harm a brand's customers and, by extension, it's reputation.

As stated, sometimes phosphate buffers are used to dry bio molecules because the water molecules inside those solutions will "dry" and immobilize those molecules to a solid surface. This method also helps to prevent not only denaturization, but other types of changes that could create trouble for the important data that someone is trying to collect.

But more than anything, phosphate buffers are about collecting as much actionable information as possible so those in leadership positions can always make the best decisions possible at all times.

Phosphate Buffer Products You Can Trust

At Weber Scientific, we always make it a priority to offer a wide range of different phosphate buffer products and solutions for you to choose from depending on your needs. One of those is our Weber Exclusive Buffer, 7.2pH Stock Dilution, which is specially prepared to buffer your dilution water blanks necessary to perform Standard Plate Count tests for bacteria. Instead of going through the long and arduous process normally associated with this task, all the work has been done for you. The best part of all is that this product works with just one liter of MS water.

Another example is the Mega-Reg 400 (TM) Butterfield's Phosphate Buffer, a Weber Exclusive. This product was designed to be a cost-effective and convenient way to aid in the sampling of chicken carcasses for all necessary generic E. coli testing.

Weber Scientific Dilution Bottles

Weber DB Sterilized Pre-filled Dilution Bottles are a convenient and eco-friendly phosphate buffer option for your laboratory. Each bottle is pre-filled, has a capacity of 150 mL, and is recyclable. Additionally, Weber DB bottles are the only PET disposable dilution bottles on the market, sporting glass-like clarity. This allows you to visually inspect the contents while also knowing that they will remain safely contained if dropped.

Weber DB™ Dilution Bottles are pre-filled to either 99 or 90 mL ±2 mL and buffered to pH 7.2 ±0.2. Other important features include:

  • Natural polyethylene attached caps, a greener alternative to prior designs.
  • Sterility (there is no chance of microbial contamination) as a result of gamma irradiation.
  • Two year shelf life – the longest in the industry – from the date of manufacture.
  • Available in two formulations – Butterfield’s Buffer for food and dairy, and Phosphate Buffer for water and wastewater testing.

Weber DB™ Dilution Bottles offer a comprehensive, safe and sterile phosphate buffer option for your laboratory.

Learn About Weber Scientific

From the moment our parent company NJDL Supplies, Inc. first opened its doors all the way back in 1959 until now, our core goal has always remained the same. We aim to act as a trusted name in the industry, offering all of the phosphate buffer solutions and similar laboratory tools you need when you need them the most.

More than anything, we want to support and empower the groundbreaking work that you're doing. We can't do that if you don't trust us implicitly, and we can't get to that point if we don't offer the quality products you can depend on at the most affordable prices possible.

If you'd like to find out more information about all of our phosphate buffer options, browse online today!