OnSite® Allergen: Multiplex Tree Nut is a rapid, qualitative test designed to detect tree nut protein in rinsewater and on surfaces. The test can simultaneously detect almond, cashew, hazelnut, pecan, pistachio, and walnut residues. To a lesser degree, the test can also detect coconut residues. Results obtained using these tests can be used as part of an effective Allergen Control Plan.

Limit of Detection:	Between 1-5 mg/kg (ppm) tree nut protein in rinsewater and 10μg/ 100 cm2 on surfaces
Operation Time:	10-20 min
Suitability:	Raw and processed tree nut proteins, though detection of extensively hydrolyzed or processed allergens may be reduced. Not intended for high-analyte concentrations; signal will be faint but remain evident at 1,000 mg/kg (ppm) protein in rinsewater. For contamination levels above 1,000 mg/kg (ppm) in rinse water, false negative outcomes may occur.

 

OnSite® Allergen Multiplex Tree Nut is a test kit that includes lateral flow devices (LFDs) that detect specific food allergens in rinsewater and on environmental surfaces. This kit relies on the use of polyclonal antibodies (pAbs) directed against the target allergens. It is configured with a test line (T) and a procedural control line (C). The test line (T) will produce a red line at low to medium levels of contamination, whether that contamination is from one or several target proteins. The procedural Control Line (C) should always produce a red line, regardless of the analyte concentration. Failure for this line to appear indicates a failure of the mixture to migrate across the membrane.

To operate the kit, a rinsewater sample or surface swab is first extracted for 2 min with shaking. Then, approximately 100µL of settled sample extract is applied to the sample port, where it will begin to react with gold particles conjugated to the polyclonal antibodies. The mixture will then migrate across the membrane thus enabling visualization of the test line (T) and procedural control (C). The test outcome is interpreted by visualizing the appearance of these lines at 10 or 15 minutes based on desired LOD.

Interpretation of Results:

 

This test is intended for laboratory and industry use, including within food production facilities, commercial kitchens, contract laboratories, and auditing programs. Thes tests should only be performed by trained personnel. OnSite® Allergen Multiplex Tree Nut kits are not suitable for home/consumer use.

Kit Components: OnSite® LFD Devices, Single-Use Droppers, Breakaway Swabs, Pre-Measured OnSite® LFD Extraction Buffer, Instructions for Use

Key Features

• Test works on surfaces and rinsewater
• Simultaneously detects almond, cashew, hazelnut, pecan, pistachio, walnut
• Detects as little as 1-5 mg/kg (ppm) of tree nut protein in rinsewater or 10μg/ 100 cm² on surfaces
• Requires no equipment
• Results in less than 20 minutes
• Sensitive to coconut protein at levels above 50 mg/kg (ppm)