M-Endo Agar LES (Difco)
m Endo Agar LES is used for enumerating coliforms in water by membrane filtration.
Principles m Endo Agar LES contains peptones as sources of carbon, nitrogen, vitamins and minerals. Yeast extract supplies B-complex vitamins, which stimulate bacterial growth. Lactose is the carbohydrate. Phosphates are buffering agents. Sodium chloride maintains the osmotic balance of the medium. Sodium desoxycholate and sodium lauryl sulfate are added as inhibitors. Basic fuchsin is a H indicator. Sodium sulfite is added to decolorize the basic fuchsin solution. Agar is the solidifying agent.
Lactose-fermenting bacteria produce acetaldehyde that reacts with the sodium sulfite and fuchsin to form red colonies. The development of a metallic sheen occurs when the organism produces aldehydes with the rapid fermentation of lactose. If the inoculum is too heavy, the sheen will be suppressed. Lactose-nonfermenting bacteria form clear, colorless colonies.