Listeria Enrichment Broth (Modified UVM I)

The enrichment broth UVW (University of Vermont) involves a two-step process, leading to a higher degree of isolation of Listeria monocytogenes in meat products (including poultry). UVM 1 enrichment broth allows for primary enrichment of Listeria.

Principles

• Peptones, yeast extract and meat extract supply the essential nutrients for the growth of
• Sodium chloride concentration increases selectivity
• Phosphates buffer the pH of the medium
• Esculin is hydrolyzed to glucose and esculetin by Listeria
• Nalidixic acid blocks the DNA replication of bacteria sensitive to this antibacterial agent
• Accompanying gram-positive microflora are inhibited by acriflavine