EE Broth acc. to Mossel
EE Broth, buffered and containing bile salts, brilliant green and glucose, proposed by Mossel, is selective enrichment media for the enumeration by Most Probable Number of Enterobacteriaceae in food, animal, and environmental products. I tis also recommended in the various harmonized Pharmacopeia for the detection of Gram-negative bacteria that are resistant to bile salts. The typical composition is that found in the European Pharmacopeia.
Principles
- The simultaneous presence of bile salts and brillant green leads to the inhibition of nearly all gram-positive bacteria as well as gram-negative stains other than enterobacteria.
- Pancreatic digest of gelatin and glucose constitute the Nitrogen and energy sources, respectively, for the development of Enterobacteriaceae
- Phosphates all ow a suitable buffering of the media in order to increase its capacity for recuperation
