Buffered Peptone Water

Buffered Peptone Water is used for pre-enriching damaged Salmonella spp. from various food sources.

Principles

Edel and Kampelmacher noted that food preservation techniques such as heat, desiccation, high osmotic pressures, or pH changes can cause sub-lethal injury to Salmonella microorganisms. Pre-enrichment with Buffered Peptone Water allows for the repair of cell damage by maintaining a high pH over the pre-enrichment period due to the presence of Mono- and Disodium Phosphate. The high pH capacity is especially useful for vegetable samples, which have a low buffering capacity.

3120-46, 3120-47 Technical Data Sheet

Alpha Bio BPW Data Sheet

3116-13 BPW SDS

Weber Scientific stocks buffered peptone water from leading suppliers including BIOKAR Diagnostics and Alpha Biosciences. Food safety labs use BPW as the foundation for detecting heat-damaged and stressed Salmonella. The phosphate-buffered formula maintains pH 7.0 throughout pre-enrichment, allowing injured bacterial cells to recover. Common applications include poultry processing, vegetable testing, and dairy quality control. Available in 500g, 2kg, and 5kg sizes. Sign in to your account or contact your sales rep for organizational pricing.

Frequently asked questions

Why buffered vs standard peptone?

The phosphate buffer maintains pH 7.0 during incubation, improving recovery of injured organisms.

What testing protocols use BPW?

USDA and FDA food safety protocols for Salmonella detection.

What sizes are available?

500g, 2kg, and 5kg.