Azure Biosystems Cielo 6 Real-Time PCR System

The Azure Biosystems Cielo 6 Real-Time PCR System was designed specifically for use in multiplex experiments involving as many as six different targets. Innovative optical technology that consists of two sets of sixteen (16) optical fibers enables the imaging of sixteen (16) individual wells at the same time. This makes it possible to image an entire 96-well plate using all six fluorescence channels in under nine (9) seconds. In addition, the Azure Cielo Real-Time PCR system utilizes a total of six unique fluorescence channels that cover most of the qPCR chemical spectrum. The channel-specific excitation and emission light emitting diode (LED) light sources ensure that specific wells on the qPCR plate are illuminated. This results in an increase in sensitivity while simultaneously minimizing the amount of background noise.

The Azure Cielo PCR System was developed using a Peltier-based thermal block system for superior thermal cycling and an unsurpassed optical design to ensure that every run is accurate and reproducible. Over the span of at least 1000 qPCR runs, the optics and thermal block of the Cielo equipment delivered the same level of uniformity and reproducibility, giving users confidence in the instrument's dependability.

Key Features

  • Engineered to be used in a diverse range of qPCR applications offering exceptional sensitivity and a broad dynamic range
  • User interface allows simple touchscreen assay configuration and saved protocols make assay set up easy and reproducible
  • qPCR data can be automatically delivered to your email inbox following a run. Cielo facilitates seamless data transfer using Ethernet, Wi-Fi, or USB connections.
  • Quickly set up and confirm plate layout by coloring each well by sample type, biological replicate, and technical replicate. Well type and standard curve values are also easily visible.
  • Scans multiple data points within each well eliminating the need for normalization dyes such as ROX
  • Compatible with low-profile, non-skirted or semi-skirted rimless 96-well plates and low-profile tubes (attached or individual) with flat caps

Azure Cielo Manager

The Azure Cielo Manager is PC-compatible software designed to facilitate the configuration, execution, and analysis of real-time PCR data.

  • Data analysis modes for qPCR with dye/SYBR, quantitative PCR with probe, allele discrimination with probe, comparative quantitation
  • Intuitive plate map design allows users to assign well ID as standards, unknown, calibrators, negative controls, NTC, etc
  • Easily set up and monitor thermal profiles including temperature, time, and plate scan for each step
  • View real-time qPCR graphs during an active experiment to monitor real-time reactions
  • Post-run summary and analysis with ability to view statistical data in the form of charts, curves, and graphs
  • View a complete and easily customizable report of the qPCR experiment that can be readily exported
  • Easily copy/paste data or graphs into Paint, Microsoft Office, or other supported applications as needed
  • Export data to MS Office, PDF, or MIQE preferred RDML (1.0, 1.1, 1.2) formats

Compatible Dyes/Probes
FAM™CAL Fluor® Red 610
CAL Fluor® 540Liz®
CAL Fluor® Orange 530Mustang Purple®
Cy3Quasar 705

Specifications for Azure Cielo 6 Real-Time PCR System
Sample Capacity (Wells)96
Compatible Plates/TubesLow-profile, semi or non-skirted, 96-well plates with sealing film;
low-profile 8- or 12-well strip tubes with optical caps;
low-profile single tubes with optical caps
Reaction Volume1–150 µL (10 µL or 65 µL recommended)
Excitation SourceLED, Excitation range: 450–700nm
Emission Wavelengths510–730nm
Detection Channels6
MultiplexingUp to 6 targets
Thermal Element6 High Quality Marlow Peltier Elements
Gradient12 Independent temperature zones with
a maximum range of 40°C
Lid TemperatureHeats up to 112°C
Reaction Temperature4°C to 99.9°C
Max. Block Ramp Rate6°C/ sec
Avg. Sample Ramp Rate4°C/sec
Temperature Uniformity±0.2°C
Temperature Accuracy±0.1°C
Custom Dye/ChemistryYes
Chemistry CompatibilityFast/Standard
Detection Sensitivity1 Copy
Dynamic Range10 Logs
SensitivityDetect differences as small as 1.3-fold in target
quantities in singleplex reactions
Onboard Memory32 GB equivalent to 20,000 experiments
ConnectivityUSB, Wi-Fi, Ethernet
Electrical ApprovalsCE Certified
WxDxH12" x 20" x 17"

Item No. Product Description Unit of Measure Your Cost Purchase
Azure Cielo 6 Real-Time PCR System - 6 Channels Each
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