Paradigm Diagnostic’s STEC Broth (PDX-STEC) is intended for use in screening ground beef samples for the presence of viable Shiga toxin producing E. coli (STEC) and Salmonella species. The medium is a selective enrichment designed to promote the growth of STEC and Salmonella strains in the same enrichment broth. Following 18 hours of enrichment, crude broth samples can be streaked onto selective differential agars designed to detect and identify STEC and Salmonella spp. Broth samples, optionally, can be processed to detect virulence genes for either STEC or Salmonella via PCR analysis prior to plating onto selective differential agar. 1 Liter 3 mo. shelf life.