foodproof® Gluten Detection Kit (Biotecon Diagnostics)
The foodproof Gluten Detection Kit is designed for quick and reliable detection of gluten-containing cereals (wheat, spelt, Khorasan wheat or Kamut, rye, barley and triticale) in food samples by real-time PCR. In combination with the Allergen RM 800 reference material, quantification is also possible.
The foodproof Gluten Detection Kit provides PCR primers, hydrolysis probes (5’-nuclease probes), and convenient premixed reagents for the species specific amplification and detection of DNA of gluten-containing cereals (wheat, spelt, Khorasan wheat/Kamut, rye, barley, and triticale measured in FAM). Since only traces of gluten and the linked cereals in food can trigger severe immune responses to consumers, multi-copy targets of these cereal genomes were used to increase the sensitivity of the PCR-assay. Additionally, the Control Template and H2O PCR- grade monitor the PCR-run for validity. An internal control (measured in HEX) is used to evaluate possible PCR inhibitions by matrix effects.
In combination with the foodproof Sample Preparation Kit III (S 400 06.1) and the foodproof Magnetic Preparation Kit III (S 400 13 L), wheat, spelt, Khorasan wheat (Kamut), rye, barley, and triticale DNA can be reliable detected in difficult matrices like minced meat, spices, ice cream, and chocolate.
PCR-results are obtained within 100 minutes. Optimized PCR conditions allow the analysis of the specific PCR of gluten-containing cereals in a single run. The foodproof Gluten Detection Kit is specifically adapted for PCR using real-time PCR instruments.