Trypto Soy Agar with Lecithin and Tween 80 is a general growth medium prepared with or without enrichments. It is used for the detection and enumeration of microorganisms on surfaces where the monitoring of sanitation is important.

Principles Enzymatic Digest of Casein and Soy Peptone provide amino acids, peptides, and other nitrogenous substances. Sodium Chloride provides essential electrolytes and maintains the osmotic equilibrium. Dextrose is the fermentable carbohydrate. Dipotassium Phosphate serves as pH buffer. Lecithin is added to neutralize quaternary ammonium compounds, while Tween (polysorbate) 80 neutralizes phenols, hexachlorophene, formalin and, combined with lecithin, ethanol. Agar is the gelifying agent.