Enterolert® Rapid Enterococci Test (IDEXX)
Enterolert detects and quantifies enterococci in just 24 hours. Sample fluoresces to indicate the presence of enterococci. Eliminate tedious membrane filtration work. Enterolert is an official ASTM Method (#D6503-99).
Shelf life: Up to 18 months at 4-30°C.
Enterolert has a significantly lower rate of false positives and false negatives than membrane filtration, giving it a sensitivity of 99.6% and a specificity of 94.9%, as indicated under the Specs Tab.
How to Use Enterolert Quantification
Step 1. Add reagent to sample.
Step 2. Pour into Quanti-Tray® (counts from 1-200) or Quanti-Tray®/2000 (counts from 1-2,419).
Step 3. Seal in Quanti-Tray® Sealer and incubate for 24 hours at 41°C.
Step 4. Count fluorescent wells and refer to most probable number (MPN) table.
Enterolert vs. Membrane Filtration (MF)
|False + Rate||10.00%||5.10%|
|False – Rate||11.70%||0.40%|
|Hands-on Time||16 min.||2 min.|
|Incubation||48 hrs. + 20 min.||24 hrs.|
May, 1995, ASM Poster Presentation, State of Connecticut, Dept. of Public Health, Gary Budnick, et al.